Composition of the Stabilizer and Conditions of Lyophilization for Preserving Infectious Activity of Influenza Virus

In this study, protective media containing no animal proteins and lyophilization regimen were determined. The optimum lyophilization regimen consisted of three stages: freezing at -70 °C, main stage at -35°C, and drying at 24°C. Protective medium containing 4% trehalose or protective medium with 10% sucrose and 5% soy peptone ensured highest stability of the lyophilized vaccine preparation in temperature range of 4-24°C. This can help to overcome possible break in the cold ch ain, which is important during transporting or storage of vaccine preparations.
Source: Bulletin of Experimental Biology and Medicine - Category: Biology Source Type: research