Selection of inactivation medium for fungal spores in clinical wastes by supercritical carbon dioxide.

Selection of inactivation medium for fungal spores in clinical wastes by supercritical carbon dioxide.
Environ Sci Pollut Res Int. 2018 May 21;:
Authors: Noman E, Norulaini Nik Ab Rahman N, Al-Gheethi A, Nagao H, Talip BA, Ab Kadir O
Abstract
The present study aimed to select the best medium for inactivation of Aspergillus fumigatus, Aspergillus spp. in section Nigri, A. niger, A. terreus var. terreus, A. tubingensis, Penicillium waksmanii, P. simplicissimum, and Aspergillus sp. strain no. 145 spores in clinical wastes by using supercritical carbon dioxide (SC-CO2). There were three types of solutions used including normal saline, seawater, distilled water, and physiological saline with 1% of methanol; each solution was tested at 5, 10, and 20 mL of the water contents. The experiments were conducted at the optimum operating parameters of supercritical carbon dioxide (30 MPa, 75 °C, 90 min). The results showed that the inactivation rate was more effective in distilled water with the presence of 1% methanol (6 log reductions). Meanwhile, the seawater decreases inactivation rate more than normal saline (4.5 vs. 5.1 log reduction). On the other hand, the experiments performed with different volumes of distilled water (5, 10, and 20 mL) indicated that A. niger spores were completely inactivated with 10 mL of distilled water. The inactivation rate of fungal spores decreased from 6 to 4.5 log as the amount of distilled water increase...

Source: Environmental Science and Pollution Research International - May 21, 2018 Category: Environmental Health Authors: Noman E, Norulaini Nik Ab Rahman N, Al-Gheethi A, Nagao H, Talip BA, Ab Kadir O Tags: Environ Sci Pollut Res Int Source Type: research