Tissue-selective alteration of ethanolamine plasmalogen metabolism in dedifferentiated colon mucosa

Publication date: August 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Volume 1863, Issue 8 Author(s): Daniel H. Lopez, Joan Bestard-Escalas, Jone Garate, Albert Maimó-Barceló, Roberto Fernández, Rebeca Reigada, Sam Khorrami, Daniel Ginard, Toshiro Okazaki, José A. Fernández, Gwendolyn Barceló-Coblijn Human colon lipid analysis by imaging mass spectrometry (IMS) demonstrates that the lipid fingerprint is highly sensitive to a cell's pathophysiological state. Along the colon crypt axis, and concomitant to the differentiation process, certain lipid species tightly linked to signaling (phosphatidylinositols and arachidonic acid (AA)-containing diacylglycerophospholipids), change following a rather simple mathematical expression. We extend here our observations to ethanolamine plasmalogens (PlsEtn), a unique type of glycerophospholipid presenting a vinyl ether linkage at sn-1 position. PlsEtn distribution was studied in healthy, adenomatous, and carcinomatous colon mucosa sections by IMS. In epithelium, 75% of PlsEtn changed in a highly regular manner along the crypt axis, in clear contrast with diacyl species (67% of which remained constant). Consistently, AA-containing PlsEtn species were more abundant at the base, where stem cells reside, and decreased while ascending the crypt. In turn, mono−/diunsaturated species experienced the opposite change. These gradients were accompanied by a gradual expression of ether li...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - Category: Lipidology Source Type: research