Hydroxysafflor Yellow A Protects Brain Microvascular Endothelial Cells against Oxygen Glucose Deprivation/Reoxygenation Injury: Involvement of Inhibiting Autophagy via Class I PI3K/Akt/mTOR Signaling Pathway.

Hydroxysafflor Yellow A Protects Brain Microvascular Endothelial Cells against Oxygen Glucose Deprivation/Reoxygenation Injury: Involvement of Inhibiting Autophagy via Class I PI3K/Akt/mTOR Signaling Pathway.
Brain Res Bull. 2018 May 15;:
Authors: Yang G, Wang N, Seto SW, Chang D, Liang H
Abstract
The present study aimed to test whether Hydroxysafflor yellow A (HSYA) protects the brain microvascular endothelial cells (BMECs) injury induced by oxygen glucose deprivation/reoxygenation (OGD/R) via the PI3K/Akt/mTOR autophagy signaling pathway. Primary rat BMECs were cultured and identified by the expression of factor VIII-related antigen before being exposed to OGD/R to imitate ischemia/reperfusion (I/R) damage in vitro. The protective effect of HSYA was evaluated by assessing (1) cellular morphologic and ultrastructural changes; (2) cell viability and cytotoxicity; (3) transendothelial electrical resistance (TEER) of monolayer BMECs; (4) cell apoptosis; (5) fluorescence intensity of LC3B; (6) LC3 mRNA expression; (7) protein expressions of LC3, Beclin-1, Zonula occludens-1 (ZO-1), phospho-Akt (p-Akt), Akt, phospho-mTOR (p-mTOR) and mTOR. It was found that HSYA (20, 40, and 80 μM) and 3-MA effectively reversed the cellular morphological and ultrastructural changes, increased cell survival, normalized the permeability of BMECs, and suppressed apoptosis induced by OGD/R (2 h OGD followed by 24 h reoxygenation). Concurrently, HSYA a...

Source: Brain Research Bulletin - May 15, 2018 Category: Neurology Authors: Yang G, Wang N, Seto SW, Chang D, Liang H Tags: Brain Res Bull Source Type: research