GSE110350 Role of endogenous type I Interferon in spontaneous, Her2/Neu oncogene-driven carcinogenesis

Contributors : Luciano Castiello ; Eleonora Aric ò ; Paola Sestili ; Filippo Belardelli ; Enrico Proietti ; Giovanna Schiavoni ; Domenica Monque ; Antonella Sistigu ; Rosanna Dattilo ; Fiorella Ciaffoni ; Mauro Biffoni ; Maria Ferrantini ; Manuela Iezzi ; Federica Moschella ; Alessi Lamolinara ; Ann Zeuner ; Anna M Pacca ; Daniele MacchiaSeries Type : Expression profiling by arrayOrganism : Mus musculusType I Interferons (IFN-I) are anti-viral and immuno-modulating cytokines involved in many steps across tumor initiation and progression. IFN-I act directly on tumor cells inhibiting cell growth and indirectly by activating immune cells to mount antitumor response. To understand the role of endogenous IFN-I in spontaneous, oncogene-driven carcinogenesis, we characterized tumors arising in Her2/neu transgenic (neuT) mice carrying a non-functional mutation in the IFN-I receptor (IFNAR1), thus being unresponsive to this family of cytokines. Compared to parental neu+/-mice (neuT mice), IFNAR1-/- neu+/- mice (IFNAR-neuT mice) showed earlier onset and increased tumor multiplicity with marked vascularization. Of note, IFNAR-neuT tumors specifically exhibited deregulation of genes having adverse prognostic value in breast cancer patients, including breast cancer stem cells (BCSC) marker aldehyde dehydrogenase-1A1 (ALDH1A1). An increased amount of BCSC was observed in IFNAR-neuT tumors, as assessed by ALDH1A1 enzymatic activity, clonogenic assay and tumorigenic capac...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research