Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation

Publication date: Available online 19 May 2018 Source:Stem Cell Research Author(s): Svitlana Malysheva, Stephanie Wunderlich, Alexandra Haase, Gudrun Göhring, Ulrich Martin, Sylvia Merkert Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as a human in vitro tool to study human trophoblast and intestinal differentiation.
Source: Stem Cell Research - Category: Stem Cells Source Type: research