Unveiling the fungal biotransformation of hydralazine using 13C-precursor

Publication date: August 2018 Source:Phytochemistry Letters, Volume 26 Author(s): Marilia O. Almeida, Adriana A. Lopes, Patricia G. Roberto, Bianca W. Bertoni, Mônica T. Pupo The fermentation of the endophyte Fusarium oxysporum SS46 and Fusarium oxysporum ATCC MYA 4623 with epigenetic modulator hydralazine in Czapek medium led to the isolation of 3-methyl-1,2,4-triazolo[3,4-a]phthalazine (1). The mechanism of biotransformation involves reaction with acetyl-CoA and further cyclization, and was unveiled using 13C-precursor. Additionally, AFLP technique for genomic DNA and differential gene expression (cDNA) analyses were applied for comparative profiling of the fungus under and without treatment with hydralazine. There was no change in the DNA profile of hydralazine-treated and non-treated cultures; however, cDNA-AFLP analyses showed a differential gene expression in the cultures after treatment of the fungi with hydralazine. Graphical abstract

Source: Phytochemistry Letters - May 18, 2018 Category: Chemistry Source Type: research