IJERPH, Vol. 15, Pages 1021: Analysis of Short-Term Smoking Effects in PBMC of Healthy Subjects —Preliminary Study

IJERPH, Vol. 15, Pages 1021: Analysis of Short-Term Smoking Effects in PBMC of Healthy Subjects—Preliminary Study International Journal of Environmental Research and Public Health doi: 10.3390/ijerph15051021 Authors: Joanna Wieczfinska Tomasz Kowalczyk Przemyslaw Sitarek Ewa Skała Rafal Pawliczak Early structural changes exist in the small airways before the establishment of Chronic Obstructive Pulmonary Disease (COPD). These changes are believed to be induced by oxidation. The aim of this study was to analyze the influence of short-term smoking on the expression of the genes contributing to airway remodeling and their relationship with the oxidative status of human blood cells. Blood mononuclear cells were isolated from 16 healthy volunteers and treated with cigarette smoke ingredients (CSI): nicotine, 1-Nitrosodimethylamine, N-Nitrosopyrrolidyne, vinyl chloride, acetone, and acrolein. The expression of TGF-β1, TIMP-1, SOD1, and arginase I was determined by qPCR. Additionally, thiol groups and TBARs were assessed. CSI induced TGF and TIMP-1 expression in peripheral blood mononuclear cells (PBMC), and apocynin alleviated this effect. The changes were more noticeable in the smoking group (p < 0.05). TBARs concentrations were higher in smokers, and in this group, apocynin acted more effectively. SOD1 correlated with arginase expression in smokers (p < 0.05). MMP-9 showed a significant correlation with SOD1 in both groups, bu...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research