GSE114539 Cohesin interacts with a panoply of splicing factors required for cell cycle progression and genomic organization

Contributors : Jung-Sik Kim ; Xiaoyuan He ; Jie Liu ; Zhijun Duan ; Taeyeon Kim ; Julia Gerard ; Brian Kim ; William S Lane ; William S Noble ; Bogdan Budnik ; Todd WaldmanSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensThe cohesin complex regulates sister chromatid cohesion, chromosome organization, gene expression, and DNA repair. Here we report that endogenous human cohesin interacts with a panoply of splicing factors and RNA binding proteins, including diverse components of the U4/U6.U5 tri-snRNP complex and several splicing factors that are commonly mutated in cancer. The interactions are enhanced during mitosis, and the interacting splicing factors and RNA binding proteins follow the cohesin cycle and prophase pathway of regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors results in stereotyped cell cycle arrests and alterations in genomic organization. These data support the hypothesis that splicing factors and RNA binding proteins control cell cycle progression and genomic organization via regulated interactions with cohesin and chromatin.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research