A bioorthogonal turn-on fluorescent strategy for the detection of lysine acetyltransferase activity.
We reported a panel of "turn-on" fluorescent probes for sensitive and selective detection of KAT enzymatic activity through a simple mix-and-read format. Combined with bioorthogonal substrate labelling and click chemistry, these probes produced strong "turn-on" fluorescent signals in response to KAT-mediated acylation process. This chemical biology strategy diversifies the assay toolboxes to investigate functions and mechanisms of acetyltransferase enzymes.
PMID: 29766153 [PubMed - as supplied by publisher]
Source: Chemical Communications - Category: Chemistry Authors: He M, Han Z, Qiao J, Ngo L, Xiong MP, Zheng YG Tags: Chem Commun (Camb) Source Type: research