GSE114478 Cis-regulatory divergence of eloF drives reproductive isolation in Drosophila

Contributors : Peter Combs ; Joshua Krupp ; Neil Khosla ; Dmitri Petrov ; Joel Levine ; Hunter FraserSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila sechellia x Drosophila simulansThe differential production of pheromones is a major barrier to mating between species in Drosophila. Individuals from morphologically similar sister species can produce different sets of cuticular hydrocarbons that allow potential mates to identify them as a suitable partner. In order to elucidate cis-regulatory mechanisms behind speciation, we looked for allele-specific expression in hydrocarbon-producing oenocytes from F1 hybrids of the sister species D. simulans and D. sechellia. By focusing on cis-regulatory changes specific to female oenocytes, we rapidly identified a small number of candidate genes. Oour RNA-seq approach proved to be far more efficient than QTL mapping in identifying candidate genes, and it can be used to pinpoint the genetic basis for a wide range of traits differing due to cis-regulatory divergence between any interfertile species.

Source: GEO: Gene Expression Omnibus - May 16, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila sechellia x Drosophila simulans Source Type: research