Association Between Selenium and Malondialdehyde as an Efficient Biomarker of Oxidative Stress in Infantile Cardiac Surgery.

Association Between Selenium and Malondialdehyde as an Efficient Biomarker of Oxidative Stress in Infantile Cardiac Surgery.
Biol Trace Elem Res. 2018 May 12;:
Authors: de Oliveira Ulbrecht MO, Gonçalves DA, Zanoni LZG, do Nascimento VA
Abstract
The present work describes a method to quantify the level of oxidative stress in infantile cardiac surgery. Fifteen patients, 6 girls and 9 boys, aged between 3 months and 16 years were divided into three groups. The first group sought to quantify the oxidative stress from differing concentrations of selenium. The second group used malondialdehyde as an indicator of oxidative stress. Finally, the third group quantified oxidative stress by normalizing the selenium concentration via malondialdehyde. Blood aliquots of 1.50 ml, drawn from the radial artery, were collected and centrifuged for quantification of Se and MDA in plasma. The statistical method ANOVA was used with a 95% confidence interval to indicate significant statistical differences between the post- and pre-operative stage for each group. The concentrations of malondialdehyde were measured by using UV-Vis following the thiobarbituric acid reaction method. For quantification of selenium, the samples were submitted to assisted microwave digestion and measured by ICP OES. In the first two groups, it was not possible to affirm that selenium and malondialdehyde could be biomarkers of oxidative stress, so a statistic test (ANOVA) was ...

Source: Biological Trace Element Research - May 12, 2018 Category: Biology Authors: de Oliveira Ulbrecht MO, Gonçalves DA, Zanoni LZG, do Nascimento VA Tags: Biol Trace Elem Res Source Type: research