Resveratrol analogue, (E)- N -(2-(4-methoxystyryl) phenyl) furan-2-carboxamide induces G 2 /M cell cycle arrest through the activation of p53 –p21 CIP1/WAF1 in human colorectal HCT116 cells

In this study, we further discovered that CS also exerts a potent suppressive effect on HCT116 colorectal cancer cells. In contrast, normal colon cells (CCD-112 Con) were not sensitive to CS up to 72  h post treatment. CS caused cytotoxicity in HCT116 cells through several apoptotic events including activation of the Fas death receptor, FADD, caspase 8, caspase 3, caspase 9, and cleaved PARP, which occurred alongside cell cycle arrest from the up-regulation of p53 and p21. The results show that CS causes apoptosis via the activation of an extrinsic pathway leading to caspase activation and cell cycle arrest from activated p53. These findings suggest that CS may be a potential candidate for development as an anti-tumor agent in the future.
Source: Apoptosis - Category: Molecular Biology Source Type: research