Proteomic analysis of chick retina during early recovery from lens ‑induced myopia.

Proteomic analysis of chick retina during early recovery from lens‑induced myopia. Mol Med Rep. 2018 May 03;: Authors: Zhou YY, Chun RKM, Wang JC, Zuo B, Li KK, Lam TC, Liu Q, To CH Abstract Myopia development has been extensively studied from different perspectives. Myopia recovery is also considered important for understanding the development of myopia. However, despite several previous studies, retinal proteomics during recovery from myopia is still relatively unknown. Therefore, the aim of the present study was to investigate the changes in protein profiles of chicken retinas during early recovery from lens‑induced myopia to evaluate the signals involved in the adjustment of this refractive disorder. Three‑day old chickens wore glasses for 7 days (‑10D lens over the right eye and a plano lens as control over the left eye), followed by 24 h without lenses. Protein expression in the retina was measured by two‑dimensional fluorescence difference gel electrophoresis (2D‑DIGE). Pro‑Q Diamond phosphoprotein staining 2D gel electrophoresis was used to analyze phosphoprotein profiles. Protein spots with significant differences (P<0.05) were analyzed by mass spectrometry. The minus lens‑treated eye became myopic, however following 24 h recovery, less myopia was observed. 2D‑DIGE proteomic analysis demonstrated that three identified protein spots were upregulated at least 1.2‑fold in myopic recovery retinas compar...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research