Use of RNA Immunoprecipitation Method for Determining Sinorhizobium meliloti RNA-Hfq Protein Associations In Vivo.

Conclusions: This article describes the RIP method for determining Sinorhizobium meliloti RNA-Hfq associations in vivo. It is also applicable to other rhizobia living in planta, although some tissue-specific modification related to sample disruption and homogenization may be needed.
PMID: 29743820 [PubMed]

Source: Biological Procedures Online - May 12, 2018 Category: Molecular Biology Authors: Gao M, Benge A, Mesa JM, Javier R, Liu FX Tags: Biol Proced Online Source Type: research