Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies.

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies.
Biol Trace Elem Res. 2018 May 08;:
Authors: de Queiroz JV, Vieira JCS, de Oliveira G, Braga CP, da Cunha Bataglioli I, da Silva JM, de Paula Araújo WL, de Magalhães Padilha P
Abstract
Predator fish can accumulate high levels of mercury, which qualifies them as potential indicators of this toxic metal. The predatory species Brachyplatystoma filamentosum, popularly known as filhote, is among the most consumed species in the Brazilian Amazon. Continuing the metalloproteomic studies of mercury in Amazonian fishes that have been developed in the last 5 years, the present paper provides the data of protein characterization associated with mercury in muscle and liver samples of filhote (Brachyplatystoma filamentosum) collected in the Madeira River, Brazilian Amazon. The mercury concentration in the muscle and liver samples was determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein fraction was extracted in an aqueous medium, and later, a fractional precipitation procedure was performed to obtain the protein pellets. Then, the proteome of the tissue samples of this fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and a mercury mapping of the protein spots was carried out by GFAAS after acid digestion. Protein spots that...

Source: Biological Trace Element Research - May 8, 2018 Category: Biology Authors: de Queiroz JV, Vieira JCS, de Oliveira G, Braga CP, da Cunha Bataglioli I, da Silva JM, de Paula Araújo WL, de Magalhães Padilha P Tags: Biol Trace Elem Res Source Type: research