Dual functional extracellular recording using a light-addressable potentiometric sensor for bitter signal transduction.

Dual functional extracellular recording using a light-addressable potentiometric sensor for bitter signal transduction.
Anal Chim Acta. 2018 Aug 31;1022:106-112
Authors: Du L, Wang J, Chen W, Zhao L, Wu C, Wang P
Abstract
This paper presents a dual functional extracellular recording biosensor based on a light-addressable potentiometric sensor (LAPS). The design and fabrication of this biosensor make it possible to record both extracellular membrane potential changes and ATP release from a single taste bud cell for the first time. For detecting ATP release, LAPS chip was functionalized with ATP-sensitive DNA aptamer by covalent immobilization. Taste bud cells isolated from rat were cultured on LAPS surface. When the desired single taste bud cell was illuminated by modulated light, ATP release from single taste bud cells can be measured by recording the shifts of bias voltage-photocurrent curves (I-V curves) when the LAPS chip is working in discrete mode. On the other hand, extracellular membrane potential changes can be monitored by recording the fluctuation of LAPS photocurrent when the LAPS chip is working in continuous mode. The results show this biosensor can effectively record the enhancive effect of the bitter substance and inhibitory effect of the carbenoxolone (CBX) on the extracellular membrane potential changes and ATP release of single taste bud cells. In addition, the inhibitory effect of CBX also confirms LAPS extracellul...

Source: Analytica Chimica Acta - May 9, 2018 Category: Chemistry Authors: Du L, Wang J, Chen W, Zhao L, Wu C, Wang P Tags: Anal Chim Acta Source Type: research