Sputum sample collected over a period of 5hours: A reliable procedure for early bactericidal activity studies

Our study was designed to test the hypothesis that an early morning sputum may be sufficient for calculation of early bactericidal activity (EBA). Patients underwent sputum collection randomly (spot, 5hours and 12hours) in consecutive days. The median CFU count in the spot samples group was 5,67 log10 CFU/mL compared to 6,17 log10 CFU/mL in 5hours and 6,23 log10 CFU/mL in 12hours samples. Inter-patient comparison showed low coefficient of variation for both 12hours (11%) and 5hours samples (10%).

Source: Diagnostic Microbiology and Infectious Disease - May 8, 2018 Category: Microbiology Authors: Cristina Paula do Nascimento, David Jamil Hadad, Luiz Guilherme Schmidt Castellani, Pedro Sousa de Almeida J únior, Reynaldo Dietze, Moises Palaci Source Type: research