Health Tip: Help Prevent E coli Infection
-- Escherichia coli(E. coli) is a bacterium that's found in the environment, foods and intestines of people and animals. Many strains are harmless, but some can make you very sick, causing diarrhea, urinary tract infection, respiratory illness and...
In this study, we sought to develop and optimise a quantitative Polymerase Chain Reaction (qPCR) assay for enumeration of hemolysin-positive UPEC in urine. Using recombinant plasmid pJET1.2::hlyD, which we termed pGU2470, the sensitivity range and linearity of amplification of qPCR was determined using primers and a probe targeting hlyD. Whole-cell preparations containing UPEC were quantified for hlyD copy number using CT values and compared to standards prepared with a known amount of pGU2470. We compared the efficiency of the assay for analysis of human and mouse urine because mouse models of human UTI are frequently use...
ConclusionsTo our knowledge, this is the first report of an E. coli ST167 strain co-producing NDM-5, CTX-M-15, and OXA-1 isolated from urine sample in China. It may help us to understand the genomic feature, molecular characteristic and resistance gene diversity of this bacterial pathogen, and the genome sequence of E. coli ECWJ1 can be used as a reference sequence for comparative analysis of NDM-5 producing E. coli aimed to reveal the mechanism of NDM-5 dissemination in this pathogen.
ConclusionThis study observed a unique phenotype of NDM-producers that has not been reported previously. The observed phenomenon poses a global threat as these pathogens may evade phenotypic screening by routine laboratories.
Conclusions: The results of the present study indicate that nitrofurantoin and fosfomycin may be considered for empirical therapy of lower UTIs in Trakya region of Turkey.
Publication date: Available online 7 August 2018Source: Veterinary MicrobiologyAuthor(s): C.A. Kennedy, C. Walsh, M. Karczmarczyk, S. O’Brien, N. Akasheh, M. Quirke, S. Farrell-Ward, T. Buckley, U. Fogherty, K. Kavanagh, C.T. Parker, T. Sweeney, S. FanningAbstractExtraintestinal pathogenic E. coli (ExPEC) possess the ability to cause extraintestinal infections such as urinary tract infections, neonatal meningitis and sepsis. While information is readily available describing pathogenic E. coli populations in food-producing animals, studies in companion/sports animals such as horses are limited. In addition, many antim...
CONCLUSION: Our findings provide possible effective drug candidates for more effective treatment of antibiotic-resistant UTIs. PMID: 30088449 [PubMed - as supplied by publisher]
This study investigated the burden and predictors of canine E. coli urinary tract infections (UTI) and antimicrobial resistance among dogs presented at a veterinary teaching hospital in South Africa, 2007 –2012.
CONCLUSION: From this study, it can be concluded that ceftriaxone + sulbactam + EDTA alone or in combination with colistin can be an effective empiric treatment of various MDR nosocomial infections and can serve as an effective alternative to carbapenems.
ConclusionsOur data indicates an escalated problem of ESBL in pediatrics with UTI, which mandates implementation of regulatory programs to reduce the spread of ESBL producing Enterobacteriaceae in the community. The use of cephalosporins, aminoglycosides (gentamicin) and trimethoprim/sulfamethoxazole is compromised in Qatar among pediatric population with UTI, leaving carbapenems and amikacin as the therapeutic option for severe infections caused by ESBL producers.
CONCLUSION: The present work confirms the inhibitory activity of the aqueous extract of edible O. corniculata and its biofabricated silver nanoparticles against urinary tract infection (UTI) causing bacteria and urolithiasis. Therefore, the consumption of O. corniculata in our daily diet may reduce the risk of UTI and urolithiasis. PMID: 30039767 [PubMed - as supplied by publisher]