Transcriptomic Profiling of Trigeminal Nucleus Caudalis and Spinal Cord Dorsal Horn.

Transcriptomic Profiling of Trigeminal Nucleus Caudalis and Spinal Cord Dorsal Horn. Brain Res. 2018 Apr 30;: Authors: Kogelman LJA, Elgaard-Christensen R, Olesen J, Jansen-Olesen I, Hansen TF Abstract The pain sensation system is highly conserved among species, thus animal models have been used to investigate relevant tissues. The focus for head-specific pain has been on the primary nociceptive neurons in the trigeminal pathway, i.e. trigeminal ganglia. The secondary nociceptive neurons of the trigeminal pathway, trigeminal nucleus caudalis (TNC), have not been assessed. We expect different gene expression profiles compared to the homologous spinal cord dorsal horn (SDH), as several signalling substances provoke head-specific pain but not peripheral pain. We aim to provide expression profiles of TNC and SDH, tissues highly relevant for pain- and migraine-studies. We extracted RNA from laser capture microdissected laminae I-V from TNC and SDH from six Wistar rats for RNA-Sequencing. We showed the expression profiles of genes involved in neural signal transmission and found that among all G protein-coupled receptors Gabbr1 was highest expressed in both tissues. Among the migraine-associated genes we showed that Cacna1a, where non-synonyms mutations can cause familial hemiplegic migraine, was highly expressed with a slightly lower expression in TNC than in SDH. To show the genetic differences between the two homologous systems we perfo...
Source: Brain Research - Category: Neurology Authors: Tags: Brain Res Source Type: research