Fibroblast Compression and Tumor Cells Migration

Role of Compression in MetastasisOurHuman Pancreatic Fibroblasts play a key role in this study.Pancreatic fibroblasts are continuously gaining ground as an important component of tumor microenvironment that dynamically interact with cancer cells to promote tumor progression. In addition, these tumor-infiltrated fibroblasts can acquire an activated phenotype and produce excessive amounts of extracellu lar matrix creating a highly dense stroma, a situation known as desmoplasia. Maria Kalli, Panagiotis Papageorgis, Vasiliki Gkretsi, Triantafyllos Stylianopoulos. (2018).Solid Stress Facilitates Fibroblasts Activation to Promote Pancreatic Cancer Cell Migration. Annals of Biomedical Engineering. https://doi.org/10.1007/s10439-018-1997-7.FIGURE 1. A schematic of the in vitro transmembrane pressure device. (a) Fibroblasts were grown as a monolayer on the transmembrane of a 0.4 lm transwell insert and a piston of adjustable weight was applying a compressive stress. Control cells were covered with an agarose cushion only. (b) The experimental set-up of the co-culture system consisted of fibroblasts and pancreatic cancer cells (MIA PaCa-2 or CFPAC-1) in the upper and lower chamber of a transwell insert, respectively. A piston with adjustable weight, applying 4.0 mmHg of compressive stress on fibroblasts for 48 h is shown. A co-culture system consisting of fibroblasts and cancer cells without a compressive load was used as a control.FIGURE 2. Neuromics '  Human Pancreatic Fibrob...
Source: Neuromics - Category: Neuroscience Tags: Cancer Associated Fibroblasts Desmoplasia Human Pancreatic Fibroblasts metastasis Source Type: news