Role of interleukin-32 in the pathogenesis of endometriosis: in vitro, human and transgenic mouse data

This study included comparison of IL-32 levels in the peritoneal fluids between women with and without endometriosis,in-vitro experiments using Ishikawa cells and endometrial stromal cells (ESCs), and experiments on IL-32 transgenic mice and wild-type mice with induced endometriosis.PARTICIPANTS/MATERIALS, SETTING, METHODSIL-32 levels in the peritoneal fluids were measured using enzyme-linked immunosorbent assays. Cell viability, expression of proliferating cell nuclear antigen (PCNA), and cellular invasiveness were analyzed followingin-vitro treatment of Ishikawa cells and ESCs with recombinant IL-32 alpha ( α) and gamma (γ). Ectopic endometriotic lesions were compared between IL-32 transgenic mice and wild-type mice after autologous endometrial transplantation with immunohistochemistry for Ki-67 antigen and PCNA.MAIN RESULTS AND THE ROLE OF CHANCEThe peritoneal fluid concentration of IL-32 was significantly higher in patients with advanced stage endometriosis compared with the controls.In-vitro treatment with IL-32 α and γ caused significant increases in cellular viability, PCNA expression, and invasiveness in Ishikawa cells and ESCs. The IL-32 transgenic mice had a significantly larger size of the ectopic endometrial lesions with higher expression of Ki-67 antigen and PCNA compared with wild-type mice.LARGE SCALE DATAN/ALIMITATIONS, REASONS FOR CAUTIONIt is still unclear whether IL-32 is a main regulator, or one of several downstream proinflammatory cytokines, causing ...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research