Aberrant detection of phenotypic markers in chronic lymphocytic leukemia (CLL) lymphocytes following cryopreservation

In a diagnostic setting, quantifying the expression of membrane proteins by flow cytometry is generally carried out using fresh blood samples where the conformation of the epitope is likely to be in its native form. However, in a research environment, cells such as peripheral blood mononuclear cells (PBMCs) are often cryopreserved in liquid nitrogen and analysed as needed. Cryogenic preservation requires the addition of cryoprotective reagents, most commonly, dimethyl sulfoxide (DMSO), in order to protect the cells from damage during the preservation process1.
Source: Experimental Hematology - Category: Hematology Authors: Tags: Brief Communication Source Type: research