Sequencing of pT5282-CTXM, p13190-KPC and p30860-NR, and comparative genomics analysis of IncX8 plasmids

Plasmids of the X incompatibility (IncX) group have been isolated dating back to the pre-antibiotic era, and the IncX group has been first identified in 1988 based on their ability to confer sensitivity to filamentous bacteriophage [1]. Sequenced IncX plasmids are predominantly from Enterobacteriaceae species, such as Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Morganella morganii and Salmonella enterica, indicating that IncX plasmids have a narrow host range. However, R6K (accession number LT827129; the archetypical plasmid of the IncX group [2]) has been shown to be transferrable from Escherichia coli to Pseudomonas aeruginosa [3] and, moreover, artificial plasmids containing the R6K-derived replicons are able to stably replicate in phylogenetically diverse species from Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria [4].

Source: International Journal of Antimicrobial Agents - April 18, 2018 Category: Drugs & Pharmacology Authors: Haihong Fang, Jiao Feng, Yuanhong Xu, Ying Zhang, Zhe Zhan, Zhe Yin, Xiongbo Duan, Bo Gao, Yigang Tong, Jinglin Wang, Yanping Luo, Erhei Dai, Dongsheng Zhou Source Type: research