The CD163 long-range scavenger receptor cysteine-rich repeat: expression, purification and X-ray crystallographic characterization

In this study, the CD163 long-range SRCR repeat was expressed in Drosophila Schneider 2 cells. The recombinant protein was homogeneous after purification by metal-affinity, cation-exchange and size-exclusion chromatography. Single crystals were obtained using 20% PEG 4000, 0.15   M potassium sodium tartrate tetrahydrate pH 8.5 and diffracted to 3.30   Å resolution. As the first view of a long-range SRCR repeat, this work lays the structural basis for a deep understanding of SRs and their multiple functions.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: scavenger receptor long-range scavenger receptor cysteine-rich repeat SRCR repeat CD163 X-ray crystallography research communications Source Type: research