Structure of the tandem PX-PH domains of Bem3 from Saccharomyces cerevisiae

The structure of the tandem lipid-binding PX and pleckstrin-homology (PH) domains of the Cdc42 GTPase-activating protein Bem3 from Saccharomyces cerevisiae (strain S288c) has been determined to a resolution of 2.2   Å (Rwork = 21.1%, Rfree = 23.4%). It shows that the domains adopt a relative orientation that enables them to simultaneously bind to a membrane and suggests possible cooperativity in membrane binding.

Source: Acta Crystallographica Section F - April 24, 2018 Category: Biochemistry Authors: Ali, I. Eu, S. Koch, D. Bleimling, N. Goody, R.S. M ü ller, M.P. Tags: PX domain PH domain phox pleckstrin homology phosphatidylinositol phosphates PIP Saccharomyces cerevisiae Bem3 research communications Source Type: research