Characterization of the small intestinal lesion in celiac disease by label-free quantitative mass spectrometry.

In this study differences in global protein expression of small intestinal biopsies from CD patients were quantified by analyzing formalin-fixed, paraffin-embedded material using liquid-chromatography mass spectrometry and label-free protein quantitation. Protein expression was compared in biopsies collected from the same patients before and after one year treatment with gluten-free diet (n=10) or before and after three-day gluten provocation (n=4). Differential expression of proteins in particular from mature enterocytes, neutrophils, and plasma cells could distinguish untreated from treated CD mucosa, and immunoglobulin variable region IGHV5-51 expression was found to serve as a CD-specific marker of ongoing immune activation. In patients that had undergone gluten challenge coordinated up-regulation of wound response proteins including the CD autoantigen transglutaminase 2 was observed. Our study provides a global and unbiased assessment of antigen-driven changes in protein expression in the celiac intestinal mucosa. PMID: 29684362 [PubMed - as supplied by publisher]
Source: Am J Pathol - Category: Pathology Authors: Tags: Am J Pathol Source Type: research