Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury.

Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury. J Immunol Res. 2018;2018:6879183 Authors: Pinheiro AJMCR, Gonçalves JS, Dourado ÁWA, de Sousa EM, Brito NM, Silva LK, Batista MCA, de Sá JC, Monteiro CRAV, Fernandes ES, Monteiro-Neto V, Campbell LA, Zago PMW, Lima-Neto LG Abstract The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100-300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-α and IL-1β expression in comparison with vehicle controls (p < 0.05). Additionally, incubat...
Source: Journal of Immunology Research - Category: Allergy & Immunology Tags: J Immunol Res Source Type: research