Superiority of rat over murine model for studies on the evolution of cancer genome.

Superiority of rat over murine model for studies on the evolution of cancer genome. Free Radic Res. 2018 Apr 18;:1-131 Authors: Akatsuka S, Li GH, Toyokuni S Abstract Evolution of the species and carcinogenesis are similar in that genomic alterations are the key events. Oxidative stress derived from various etiologies is one of the major causes of carcinogenesis by inducing mutations in the genome. Persistent oxidative stress in the renal proximal tubules through Fenton reaction catalyzed by ferric nitrilotriacetate (Fe-NTA) generates renal cell carcinoma (RCC) in mice and rats. Here, in order to observe the species difference in oxidative stress-induced carcinogenesis and to obtain an insight regarding the characteristics of each species, we compared the genomic alterations using array-based comparative genome hybridization among RCCs in Mutyh knockout/wild-type mice (C57BL/6 background) induced by Fe-NTA, RCCs in F1 hybrids of Brown-Norway/Fischer-344 wild-type rats and clear cell renal cell carcinoma (CCRCC)/papillary renal cell carcinoma (PRCC) of humans. The average deviated fraction of genomic segments, either loss or gain, from the standard biallelic position was 0.220 (N = 4), 0.304 (N = 11), 0.283 (N = 12) and 0.261 (N = 5), respectively, for murine RCC, rat RCC, human CCRCC and human PRCC. Notably, gain/loss ratio was remarkably different as indicated by 0.0820, 0.161, 0.821 and 4.44, respectively. These data sugges...
Source: Free Radical Research - Category: Research Tags: Free Radic Res Source Type: research