GSE104487 Effect of β-adrenergic receptor antagonist and agonist on cardiac structure and function and whole body gene expression in Daphnia magna

Contributors : Tae-Yong Jeong ; Jana Asselman ; Karel A De Schamphelaere ; Filip Van Nieuwerburgh ; Dieter Deforce ; Sang D KimSeries Type : Expression profiling by high throughput sequencingOrganism : Daphnia magnaPropranolol, a human β-adrenergic receptor antagonist, is an active pharmaceutical ingredients (APIs) which is abundant in aquatic environments and reactive in non-targeted organism. Because propranolol is considered to result in specific effects in Daphnia magna based on our previous studies, effects of β-adrenergic receptor reacting agents including both an antagonist and agonist were studied using pharmacologically relevant endpoints as well as a more holistic gene expression approach to not exclude potential alternative modes of action. Isoprenaline was chosen as an agonist and cardiac structural and functio nal endpoints including heart size, contraction capacity, and heart rate were monitored. In addition, whole body gene expression profiling was performed using RNA-sequencing to provide additional information on mode of action (MOA). No effect was observed on heart size growth in propranolol and isop renaline exposures. Contraction capacity was decreased in isoprenaline exposure and heart rate was decreased in propranolol exposure. Time-series exposures for heart rate showed different magnitudes of effect dependent on types of chemical exposure and corresponding time-dependent differentially exp ression of gene families was detected. No significant exp...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Daphnia magna Source Type: research