904 The cytosolic dsRNA-sensing pathway mediated by MDA5/MAVS/IRF3 is critical for the induction of type I and III IFNs after viral infection of skin keratinocytes

Skin keratinocytes (KCs) are epithelial cells important for skin integrity and immunological responses. Vaccinia virus (VACV) is a large cytoplasmic DNA virus that belongs to the poxvirus family. Skin scarification with vaccinia virus has led to successful eradiation of smallpox. Vaccinia E3 is a key virulence factor composed of a Z-DNA-binding domain and a dsRNA-binding domain. We have shown that a mutant VACV lacking E3L gene (E3L) infection of murine KCs induces ifnb, Ccl4, Ccl5, and Il6 gene expression, whereas wild-type (WT) VACV does not.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Innate Immunity, Microbiology, Inflammation Source Type: research