Wash-free and selective imaging of epithelial cell adhesion molecule (EpCAM) expressing cells with fluorogenic peptide ligands.

Wash-free and selective imaging of epithelial cell adhesion molecule (EpCAM) expressing cells with fluorogenic peptide ligands.
Biochem Biophys Res Commun. 2018 Apr 13;:
Authors: Tara Bahadur KC, Suga K, Isoshima T, Aigaki T, Ito Y, Shiba K, Uzawa T
Abstract
Detection of the cells expressing an epithelial cell adhesion molecule (EpCAM) is a crucial step to identify circulating tumor cells (CTCs) from blood. To detect the EpCAM, we here designed and synthesized a series of fluorogenic peptides. Specifically, we functionalized an EpCAM-binding peptide, Ep114, by replacing its amino acids to an aminophenylalanine that was modified with environmentally sensitive 7-nitro-2,1,3-benzoxadiazole (NBD-amPhe). Among six synthesized peptides, we have found that two peptides, Q4X and V6X (X represents NBD-amPhe), retain the Ep114's binding ability and specifically mark EpCAM-expressing cells by just adding these peptides to the cultivation medium. Our wash-free, fluorogenic peptide ligands would boost the development of next generation devices for CTC diagnoses.
PMID: 29660346 [PubMed - as supplied by publisher]

Source: Biochemical and Biophysical Research communications - April 13, 2018 Category: Biochemistry Authors: Tara Bahadur KC, Suga K, Isoshima T, Aigaki T, Ito Y, Shiba K, Uzawa T Tags: Biochem Biophys Res Commun Source Type: research