Simultaneous quantification of systemic azoles and their major metabolites in human serum by HPLC/PDA: role of azole metabolic rate

We describe the development and validation of a novel liquid chromatography assay (HPLC/PDA) for simultaneous quantification of voriconazole, itraconazole, and posaconazole, as well as some of their major metabolites, voriconazole-N-oxide and hydroxy-itraconazole, in human serum. Analytes were detected using a PDA system that allows the characterization of the specific UV spectra of each compound. The assay exhibited linearity between 0.25 –16mg/L for all the compounds tested. The accuracy and within- and between-day precision of the assay were in acceptable ranges.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research