Biobeam —Multiplexed wave-optical simulations of light-sheet microscopy

by Martin Weigert, Kaushikaram Subramanian, Sebastian T. Bundschuh, Eugene W. Myers, Moritz Kreysing Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we presentbiobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105–106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
Source: PLoS Computational Biology - Category: Biology Authors: Source Type: research