Autophagy alteration prevents primary cilium disassembly in RPE1 cells.

In this study, we found that the gradual abatement in autophagy during serum-restimulation was a dynamic process and significantly correlated with the disassembly of primary cilium in human retinal pigmented epithelial (RPE1) cells. Although autophagy activity was gradually decreased during serum-restimulation, the alteration in autophagy under the same condition prevented the disassembly of the primary cilium. Autophagy inhibitors such as chloroquine, U18666A and 3-methyladenine (3-MA) retained both the number of ciliated cells and cilium length. In contrast, rapamycin treatment during serum-restimulation maintained the number of ciliated cells with shortened cilia. Taken together, alteration in autophagy during serum-restimulation prevent the disassembly of the primary cilium, and autophagy modulators may serve as useful compounds for studying mechanistic details related to the disassembly of the primary cilium and ciliopathy.
PMID: 29649478 [PubMed - as supplied by publisher]

Source: Biochemical and Biophysical Research communications - April 9, 2018 Category: Biochemistry Authors: Maharjan Y, Lee JN, Kwak S, Lim H, Dutta RK, Liu ZQ, So HS, Park R Tags: Biochem Biophys Res Commun Source Type: research