A novel immunotherapy of Brucellosis in cows monitored non invasively through a specific biomarker

We report here the successful immunotherapy of bovine Brucellosis by phage lysates of RB51 (RL) and S19 (SL). The SL induced strong antibody response and RL stimulated CMI.In vitro restimulation of leukocytes from RL immunized cattle induced interferon gamma production. A single subcutaneous dose of 2 ml of cocktail lysate (both RL and SL), eliminated live virulentBrucella from Brucellosis affected cattle with plasma level ofBrucella specific 223 bp amplicon undetectable by RT-PCR and blood negative for liveBrucella by culture in 3 monthspost-immunization. This is the first report on minimally invasive monitoring of the efficacy of antibacterial therapy employing plasma RNA specific for live bacteria as a biomarker as well as on the use of RB51 phage lysate for successful immunotherapy of Brucellosis in cattle.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research