Vimentin expression is required for the development of EMT-related renal fibrosis following Unilateral Ureteral Obstruction in mice.

Vimentin expression is required for the development of EMT-related renal fibrosis following Unilateral Ureteral Obstruction in mice. Am J Physiol Renal Physiol. 2018 Apr 04;: Authors: Wang Z, Divanyan A, Jourd'heuil F, Goldman RD, Ridge KM, Jourd'heuil D, Lopez-Soler RI Abstract Most renal transplants ultimately fail secondary to chronic allograft nephropathy (CAN). Vimentin is a member of the intermediate filament family of proteins and has been shown to be important in the development of CAN. One of the pathways leading to chronic renal fibrosis after transplant is thought to be Epithelial to Mesenchymal Transition (EMT). Even though vimentin expression is one of the main steps during EMT, it is unknown whether vimentin expression is required for EMT. To this end, the role of vimentin in renal fibrosis was determined via unilateral ureteral obstruction (UUO) in vimentin knockout mice (129 svs6 vim -/-). Following UUO, kidneys were recovered and analyzed via western blotting, immunofluorescence, and transcriptomics. Cultured human proximal renal tubular (HK-2) cells were subjected to lentiviral-driven inhibition of vimentin expression, and then treated with TGF-beta to undergo epithelial to mesenchymal transition. Immunoblotting as well as wound healing assays were used to determine development of EMT. Western blotting analyses of mice undergoing UUO reveals increased levels of vimentin soon after UUO. As expected, interstitial collagen deposition was i...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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Conclusion Inguinal herniation of the transplant ureter leading to ureteral obstruction is a rare, probably underreported, cause graft of dysfunction. Therefore, we advocate elective repair of inguinal or incisional hernias in renal transplant recipients. PMID: 31690216 [PubMed - as supplied by publisher]
Source: Acta Chirurgica Belgica - Category: Surgery Tags: Acta Chir Belg Source Type: research
We present the case of a 46-year-old man who underwent successful antegrade ureteroscopy for lithiasis in his allograft ureter. At a scheduled follow-up 15  years after transplantation, computed tomography (CT) detected a 12-mm renal stone in the renal pelvis of the transplanted kidney. During his follow-up, gross hematuria was seen; the stone moved to the ureter, causing hydronephrosis. Ultrasound and non-contrast CT revealed hydronephrosis and a 15- mm stone in the transplanted ureter. Considering the stone size, location, and the difficulty of the access to the anastomosed ureteral orifice, percutaneous ureteroscop...
Source: Urolithiasis - Category: Urology & Nephrology Source Type: research
Conclusion: In our experience, ureteroureterostomy with native ureter is technically and functionally good option for ureteric reimplantation in kidney transplant patients. It can be used selectively for elective and emergency situations where UNC is not possible. Hence, the kidney transplant surgeon should be well versed with both techniques.
Source: Indian Journal of Urology - Category: Urology & Nephrology Authors: Source Type: research
We present a case of live-related renal transplant in which complete duplication of ureter was found as a surprise during bench preparation of the donor kidney, which was reported as partial duplication on the pre-operative multidetector computed tomography. A common ostium was made from the two ureters and was anastomosed to the bladder. At 2-months follow–up, the patient has good graft function and no evidence of ureteric complications. Since double ureter does not seem to increase urological complications, such donors should not be rejected.
Source: Indian Journal of Urology - Category: Urology & Nephrology Authors: Source Type: research
In this study, we present the long-term results of tubular bladder reconfiguration and cystopyelostomy (tubular vesicopyelostomy), an innovative and practical procedure for the management of long-segment ureteric stenosis (types 2 and 3) after transplantation. Between 2002 and 2017, 722 kidney transplant patients were monitored at the University of Medical Sciences, Bozyaka Organ Transplantation and Research Center. Twenty-eight of these patients underwent tubular vesicopyelostomy operation; 17 male and 11 female patients with a mean age of 45.6 ± 10.5 years. Time to surgical intervention for ur...
Source: The International Journal of Artificial Organs - Category: Transplant Surgery Authors: Tags: Int J Artif Organs Source Type: research
We report here a series of three patients in whom the inferior vena cava as well as bilateral iliac veins were thrombosed, making it necessary to explore less traditional vessels for venous drainage of the renal allograft. We utilized the splanchnic vasculature in two cases and the native left renal vein in another. The resulting atypical intra‐abdominal locations of these allografts also presented difficulties for arterial anastomoses and for urinary drainage. Arterial conduits were utilized in two cases to facilitate anastomosis to the common iliac artery or the aorta, and in the third case, the splenic artery was used...
Source: Clinical Transplantation - Category: Transplant Surgery Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Source: Clinical Transplantation - Category: Transplant Surgery Authors: Tags: Original Article Source Type: research
CONCLUSIONS: Laparoscopic transplant is a difficult procedure that requires experience in kidney laparoscopy and laparoscopic vascular sutures. The experimental model presented is a good training option and can be used to evaluate different methods to maintain cold ischemia and to compare with the traditional open approach. PMID: 26862820 [PubMed - as supplied by publisher]
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - Category: Transplant Surgery Authors: Tags: Exp Clin Transplant Source Type: research
Conclusion: Due to the lack of transplantable organs and the long time usually spent on the waiting list, graftectomy should be only considered in case of refractory haemodynamic instability or compromised graft viability.
Source: BMC Urology - Category: Urology & Nephrology Authors: Source Type: research
Background and Aims: The aim of the study was to clarify the frequency and the sequel of surgical complications occurring within 1 year after renal transplantation. Patients and Methods: Surgical complications after 1670 consecutive adult kidney transplantations performed between 2000 and 2009 were retrospectively analyzed. In 2%, a living-related allograft was used, and 10% were retransplantations. An intravesical technique without stenting was used for the ureteric implantation. Results: There were 282 surgical complications occurring in 259 (15.5%) transplantations. Ureteral obstruction occurred in 53 (3.1%), lymphoce...
Source: Scandinavian Journal of Surgery - Category: Surgery Authors: Tags: Original Articles Source Type: research
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