High-strength double network hydrogels as potential materials for artificial 3D scaffold of cell migration in vitro

Publication date: 20 July 2018 Source:Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 549 Author(s): Xiaoting Ren, Qilin Yang, Dongguang Yang, Ying Liang, Jingjing Dong, Yueming Ren, Xili Lu, Lili Xue, Li Li, Likun Xu Cancer metastasis arises from magical pathological cell migration. An accurate and rapid evaluation of cell migration can reduce the number of animals used in experiments to develop a new drug to restrain the metastasis. In this work, high-strength double network hydrogels were synthesized to develop the three-dimensional (3D) scaffolds, which were applied to screen drug of anticancer in vitro. Compression strength was examined for Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PAMPS/PAAm) double network hydrogel, Polyethylene glycol-Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PEG-PAMPS/PAAm) double network hydrogel and Poly(vinyl alcohol)-Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)/ Polyacrylamide (PVA-PAMPS/PAAm) double network hydrogel in more than 90% deformation rate. The results showed that PEG-PAMPS/PAAm hydrogel and PVA-PAMPS/PAAm hydrogel could provide high mechanical property to afford the scaffold durability and simulate cell metastasis under a simulated blood pressure. Used as the in vitro scaffold, the PEG-PAMPS/PAAm hydrogel and PVA-PAMPS/PAAm hydrogel were co-cultured with the two cancer cell, NB4 cell and A549 cell, which is the acute promyelocytic leukemia c...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research