Polyoxygenated ergosteroids from the macrofungus Omphalia lapidescens and the structure-cytotoxicity relationship in a human gastric cancer cell line

Publication date: June 2018 Source:Phytochemistry Letters, Volume 25 Author(s): Yue Wang, Ou Dai, Cheng Peng, Hai-Guo Su, Lu-Lin Miao, Lu-Si Liu, Liang Xiong Seventeen polyoxygenated Δ22-ergosteroids (1–17), including two new compounds (1 and 15), were isolated from the ethyl acetate soluble fraction of a 95% ethanol extract of Omphalia lapidescens Schroet. According to the oxidation degree and the oxidation positions of A and B rings, the isolates could be divided into three subtypes: 3,5,6-trioxygenated Δ7-ergosteroids (1–9), 3,5,6,7-tetraoxygenated ergosteroids (10–14), and conjugated Δ4,6,8-3-one ergosteroids (15–17). The cytotoxic effects were studied using a human gastric cancer cell line, HGC-27, in vitro and the relationships between structure and cytotoxicity of the three subtypes were investigated. Among these subtypes, (3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol (5) exhibited the highest cytotoxicity, with an IC50 value of 4.17 ± 0.48 μM. Furthermore, Hoechst fluorescence 33342 staining demonstrated that compound 5 could induce HGC-27 cell apoptosis significantly. Graphical abstract
Source: Phytochemistry Letters - Category: Chemistry Source Type: research

Related Links:

ConclusionOur results show that fluoroscopic-guided NGT placement is a highly safe and effective procedure for patients with advanced HNC requiring enteral feeding.
Source: Journal of the Formosan Medical Association - Category: General Medicine Source Type: research
Lycopene induces apoptosis by inhibiting nuclear translocation of β-catenin in gastric cancer cells. J Physiol Pharmacol. 2019 Aug;70(4): Authors: Kim M, Kim SH, Lim JW, Kim H Abstract Reactive oxygen species (ROS) promote the development and progression of cancer by their effects on several signaling pathways. Lycopene, a major carotenoid natural product, is known to display antioxidant activity and to induce apoptosis of cancer cells. The aim of the present study was to investigate the mechanism by which lycopene induces apoptosis of the human gastric cancer AGS cells. In the present study, we s...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
ConclusionThe present study in Latin American countries confirmed that similar environmental factors such as smoking and grain/cereal consumption were associated with H. pylori infection and its induced gastric lesions as reported in other regions where dominant H. pylori strains differ.
Source: Cancer Epidemiology - Category: Cancer & Oncology Source Type: research
Authors: Yuan L, Ma T, Liu W, Chen Y, Yuan Q, Ye M, Yu L, Li J, Niu Y, Nan Y Abstract Emerging studies indicated that lncRNA is one crucial regulator in development and tumorigenesis. We firstly showed that LINC00994 was overexpressed in GC cells compare with GES-1. Compared to adjacent samples, the LINC00994 expression was increased in GC tissues by qRT-PCR assay. Furthermore, Elevated expression of LINC00994 induced GC cell invasion, proliferation and cycle. Luciferase reporter analysis indicated that ectopic miR-765-3p expression suppressed wild type LINC00994 luciferase activity, but not mutant LINC00994 in GC ...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
This study assessed the biological functions of LIM-domain-only 4 (LMO4) in gastric cancer (GC) and investigated the underlying molecular mechanisms. It was found that the expression of LMO4 was significantly upregulated in GC tissues and closely associated with clinicopathological factors, overall survival and disease-free survival of patients. After knockdown of LMO4 in MGC-803 and SGC-7901 cells, invasion and proliferation were obviously suppressed. Furthermore, LMO4 knockdown suppressed the phosphorylation of phosphatidylinositol 3-kinase (PI3K), Akt and mammalian target of rapamycin (mTOR). Miltefosine, the inhibitor ...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Conclusions: Similar long-term outcomes were achieved in the LSKS-SRLA and LSLA-SRLA groups for locally advanced proximal gastric cancer. However, in the aspects of surgical time, length of incision, and postoperative recovery, the LSKS-SRLA group had obvious advantages. PMID: 31737066 [PubMed]
Source: Gastroenterology Research and Practice - Category: Gastroenterology Tags: Gastroenterol Res Pract Source Type: research
Authors: Yanai H, Chihara D, Harano M, Sakaguchi E, Murakami T, Nishikawa J Abstract A 73-year-old man visited our hospital for the treatment of an early gastric cancer (GC) lesion. We performed en bloc resection using endoscopic submucosal dissection (ESD) for his GC lesion. The present GC lesion was Epstein-Barr virus (EBV)-associated poorly differentiated-type adenocarcinoma invading into the submucosal layer. Recently, accumulating data has shown that the risk of lymph node metastasis from early EBV GC without local lymphovascular infiltration is low. The present patient has been in good health for over three y...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
This article presents a review of literature published in the past 15 years. H. pylori-mediated persistent methylation levels are associated with the development of metachronous gastric cancer. The methylation of certain specific genes can be used to identify patients with a high risk of metachronous gastric cancer even after H. pylori eradication. H. pylori eradication after endoscopic resection should be performed as early as possible for eradication success and prevention of metachronous precancerous lesions. Although whether the eradication of H. pylori could prevent the development of metachronous cancer after ...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Conclusion: The diagnostic value and prognostic value of significant DEMIs in cancers of the digestive tract were identified, which may provide a novel direction for treatment and prognosis improvement of cancers of the digestive tract. PMID: 31737678 [PubMed - in process]
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Abstract Selenium has remained a controversial character in cancer research. While its antitumor effects have been widely demonstrated, further evidence is required to establish it as a robust treatment regime. Sodium selenite (SS), an inorganic selenium, reportedly affected the proliferation and redifferentiation of gastric cancer cells, but whether it could act as a complement to conventional chemotherapeutic drugs for combination therapy is uncertain. Herein, SGC-7901 and MGC-803 gastric cancer cells were treated with PADM (Ac-Phe-Lys-PABC-ADM), a prodrug of doxorubicin/adriamycin (ADM), and the combined antitu...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
More News: Cancer | Cancer & Oncology | Gastric (Stomach) Cancer | Gastroenterology