Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF- α

This study aims to confirm the effects of synoviocytes (SCs) on regulating lysyl oxidases (LOXs) and matrix metalloproteinase (MMP)-1, 2, 3 in the normal and injured anterior cruciate ligament (ACL) fibroblasts response to tumor necrosis factor-α(TNF-α). The gene and protein expression levels of LOXs and MMP-1, 2, 3 in SCs cocultured ACL fibroblasts (ACLfs) induced by TNF-α and mechanical injury were analyzed by real-time polymerase chain reaction (PCR) and western bolting; the MMP-2 activity were analyzed by zymography. The results exhibited that TNF-α alone slightly downregulated the expressions of LOXs and upregulated the expression of MMP-1, 2, 3 in both normal and injured ACL fibroblasts. The decrease of LOXs and increase of MMP-1, 2, 3 in ACLfs response to TNF-α were further promoted by coculture. Taken together, these results show for the first time that the crosstalk between ACLfs and SCs could modulate the LOXs and MMP-1, 2, 3 synthesis in ACLfs in the presence of TNF-α. Accumulation of MMPs in the isolated fluid-containing space not only disrupts the balance of ACL healing, but also increases cartilage degradation and accelerates osteoarthritis (OA) in injured joint. Based on this mechanism, targeting inhibition of MMPs could provide a promising therapeutic strategy for acute ligament injury. [...] Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.Article in Thieme eJournals: Table of contents  | ...
Source: Journal of Knee Surgery - Category: Orthopaedics Authors: Tags: Original Article Source Type: research

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Authors: Zupan J, Vrtačnik P, Cör A, Haring G, Weryha G, Visvikis-Siest S, Marc J Abstract Paired cartilage and subchondral bone of subjects with no clinical history of joint disorders were analyzed to determine whether antioxidant enzymes, inflammatory cytokines and growth factors can be linked to a pre-osteoarthritis. Tissue explants were phenotyped according to Osteoarthritis Research Society International grading and micro-computed tomography, and also screened for the expression of several markers using quantitative polymerase chain reaction. The expression of these same genes was measured in SW1353 cell...
Source: Growth Factors - Category: Research Tags: Growth Factors Source Type: research
CONCLUSION: On the basis of RCTs published to date, it cannot be definitively con- cluded whether surgery or conservative (expectant) management of ACL rupture yields a better functional outcome. There is a trend in observational studies toward better functional outcomes after ACL reconstruction. As an average across studies, conservative treatment fails in 17.5% (± 15.5%) of patients. PMID: 30765021 [PubMed - in process]
Source: Deutsches Arzteblatt International - Category: General Medicine Tags: Dtsch Arztebl Int Source Type: research
CONCLUSIONS: These data suggested that our technique is a successful procedure for the trapeziometacarpal joint arthrodesis. Cross-shaped bone grafts have the advantages of restoring thumb length and providing internal stabilization, especially for rotational force. No complications arose at the bone harvest site of the iliac crest. The procedure seems to be technically demanding, particularly for adapting the bone graft to perfectly match the shape of the defect. PMID: 30760141 [PubMed - in process]
Source: Hand Surgery - Category: Surgery Authors: Tags: J Hand Surg Asian Pac Vol Source Type: research
CONCLUSIONS: Good clinical results were maintained at 5 years after surgery, but progression of postoperative wrist osteoarthritis interfered with improvement of wrist flexion. Change in the radioscaphoid angle was the factor that was most highly correlated with progression of postoperative wrist osteoarthritis. PMID: 30760138 [PubMed - in process]
Source: Hand Surgery - Category: Surgery Authors: Tags: J Hand Surg Asian Pac Vol Source Type: research
This study is registered with PROSPERO, number CRD42018105188.FindingsFrom 4363 references found by our initial search, we identified 33 case series in 30 eligible articles, which reported all-cause survival for 6490 TKRs (26 case series) and 742 UKRs (seven case series). No case series reporting on patellofemoral replacements met our inclusion criteria, and no case series reported 25 year survival for TKR. The estimated 25 year survival for UKR (based on one case series) was 72·0% (95% CI 58·0–95·0). Registries contributed 299 291 TKRs (47 series) and 7714 UKRs (five series). The pooled regist...
Source: The Lancet - Category: General Medicine Source Type: research
This study was registered with PROSPERO (CRD42018085642).FindingsWe identified 140 eligible articles reporting 150 series, and included 44 of these series (13 212 total hip placements). National joint replacement registries from Australia and Finland provided data for 92 series (215 676 total hip replacements). The 25-year pooled survival of hip replacements from case series was 77·6% (95% CI 76·0–79·2) and from joint replacement registries was 57·9% (95% CI 57·1–58·7).InterpretationAssuming that estimates from national registries are less likely to be biased, patie...
Source: The Lancet - Category: General Medicine Source Type: research
Studies of professional American football players have shown that football-related activities lead to acute injuries and may have long-term adverse health outcomes including osteoarthritis, neurocognitive impairment, and cardiovascular disease. However, th...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Occupational Issues Source Type: news
ConclusionThe injuries treated in pets in British Columbia included abscesses, sprains and abrasions. Dogs were also treated with medicinal plants for rheumatoid arthritis, joint pain and articular cartilage injuries. More than 40 plants were used. Anal gland problems were treated with Allium sativum L., Aloe vera L., Calendula officinalis L., Plantago major L., Ulmus fulva Michx., Urtica dioica L. and the lichen Usnea longissima Ach. Antioxidants in plants, such as diarctigenin from Arctium lappa, used in ethnoveterinary medicine for rheumatoid arthritis and joint pain in pets are said to be of use in treating pathologica...
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Condition:   Knee Osteoarthritis Intervention:   Device: Breg Flex Sponsors:   The Hawkins Foundation;   BREG, Inc Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Hand Osteoarthritis Interventions:   Other: Paraffin wax;   Drug: Prolotherapy Sponsor:   Bakirkoy Dr. Sadi Konuk Research and Training Hospital Active, not recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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