Peripheral blood immune response-related gene analysis for evaluating the potential risk of chronic antibody-mediated rejection.

This study aimed to elucidate the clinical relevance of immune-related gene expression in peripheral blood of kidney transplant recipients. The expression levels of fourteen key molecules (Foxp3, CTLA-4, CCR7, TGF-β, IGLL-1, IL-10, ITCH, CBLB, Bcl-6, CXCR5, granzyme B, CIITA, Baff, TOAG-1/TCAIM) related to regulatory/cytotoxic function of immune cells were compared in 93 patients, which were divided into Groups A (clinical cAMR with dn DSA, n=16), B (subclinical cAMR with dn DSA, n=17), C (negative cAMR with dn DSA, n=21) and D (stable function without dn DSA, n=39). CIITA mRNA expression levels in groups B and C were significantly lower than those in group D (p
Source: Human Immunology - Category: Allergy & Immunology Authors: Tags: Hum Immunol Source Type: research

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This study aimed to confirm whether human postmortem ASCs can be collected and culture-expanded from cadavers. Axilla subcutaneous adipose tissues were harvested during forensic autopsy and enzymatically digested to obtain a heterogeneous cell mixture, including the ASCs population. The mixture was seeded onto collagen-coated cell culture dishes and spindle-shaped adhesive and proliferative ASCs were confirmed. Senescent cells were also present, visualized as large and flattened cells. When maintained in a cool environment, ASCs were able to survive in the postmortem tissues for up to 7 days after death. We conclude that p...
Source: Journal of Forensic and Legal Medicine - Category: Forensic Medicine Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Frederick R. AppelbaumAbstractWith improvements in the safety of allogeneic hematopoietic cell transplantation, disease recurrence following the procedure is now the most frequent reason for treatment failure. Administration of maintenance therapy after transplantation is one way to try and prevent recurrence. This paper provides a brief review of the topic.
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): David P. SteensmaAbstractPatients diagnosed with myelodysplastic syndromes (MDS) often ask their physicians whether earlier detection of disease or more prompt initiation of treatment might have resulted in a better outcome. The concept of starting therapy at an early point in the disease process when the clonal burden of abnormal hematopoietic stem cells may be lower and somatic mutational complexity less, and therefore treatment more likely to be effective, is attractive. However, at present there is no ev...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Indumathy Varadarajan, Karen K. BallenAbstractAggressive curative therapies have now been extended to patients older than 65 years, a fast-growing segment of the population. As the number of allogeneic transplants in patients older than age 65 is increasing, attention is now focused on improving outcomes in this group. This paper discusses important aspects of allogeneic transplant in the older patient, focusing on donor and patient selection, choice of conditioning regimen and graft source, and the importan...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Discussion and clinical examples of potential solutions to creating a safe and effective CAR T cell for AML include: (1) Decreasing the potency or activity of CAR T cells; (2) Using transient or depleted CAR T cells as part of pre-transplant conditioning; and (3) Using a gene-edited allogeneic donor hematopoietic stem cell transplant in order to allow safe and protracted anti-AML CAR T-cell function.
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Mathew L. Cooper, John F. DiPersioAbstractAt present, the only curative therapy for patients with T-cell malignancies is allogeneic stem cell transplant, which has associated risks and toxicities. Novel agents have been tried in relapsed T-cell acute lymphoblastic leukemia (T-ALL), but only one, with 20%-30% complete remission rates, has been approved by the US Food and Drug Administration. T-ALL is a heterogeneous disease, but it has universal overexpression of CD7 as well as several other T-cell markers, s...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Hrishikesh K. Srinagesh, James L.M. FerraraAbstractAcute graft-versus-host disease (GVHD) is the major complication of allogeneic hematopoietic cell transplantation and is the primary cause of early non-relapse mortality (NRM) after transplant. GVHD of the gastrointestinal (GI) tract fuels the systemic inflammatory reaction and consequently is the principal driver of mortality. Recently, the MAGIC algorithm probability (MAP) that is computed from two biomarkers of GI GVHD has been validated to accurately pre...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Nelson J. ChaoAbstractAllogeneic hematopoietic stem cell transplantation (HSCT) has the potential for providing a cure for several hematologic malignancies. Although in most circumstances, allogeneic HSCT is preceded by disease-directed or cytoreductive therapy, it is unclear if these toxic conditioning regimens can be circumvented. This review summarizes evidence that will provide insights into factors that influence outcomes in allogeneic HSCT and whether this curative therapy could be used right at diagnosis.
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Benjamin A. Derman, Richard A. LarsonAbstractRecent advances in remission induction treatment strategies for acute myeloid leukemia (AML) have improved the rates of complete remission (CR) and overall survival (OS), owing to a concerted effort to tailor therapies toward specific AML subtypes. However, without effective post-remission therapy, most patients will relapse. The extent to which post-remission therapies is individualized in the current paradigm is quite varied. Core binding factor (CBF) AML is typ...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
A 39-year-old male, live-related renal transplant recipient with stable graft function presented with multiple painless, non-itchy, papular lesions over the midback region. Three months later, these lesions became multiple abscesses with pus exudation (Figure  1). The patient had a history of injury to his midback from the sharp edge of cement stairs, which caused an abrasion. He had received a thymoglobulin induction and was on prednisolone, tacrolimus, and azathioprine. The microscopic examination of KOH mount of pus revealed multiple, septate, branch ing, brown-pigmented hyphae with thick-walled vesicular swellings...
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Nephrology Image Source Type: research
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