Suppression of TLR4/NF- κB Signaling Pathway Improves Cerebral Ischemia–Reperfusion Injury in Rats

AbstractThe present study aimed to investigate the effects of the toll-like receptor 4 (TLR4)/nuclear factor-kappaB (NF- ҡB) signaling pathway in cerebral ischemia–reperfusion injury. A total of 125 male Sprague Dawley (SD) rats were selected for this study. Fifty SD rats were randomly divided into the control, sham injury, 0.5 h perfusion, 2 h perfusion, and 6 h perfusion groups to establish the model of ischem ia–reperfusion. The rat brain injury model was established using the other 75 male SD rats, and different model groups with different treatments were established (15 rats per group): model control group (injected with 10 μL of saline solution), model + TAK-242 group (injected with resatorvid), m odel + PDTC group (injected with pyrrolidine dithiocarbamate), model + LPS group (injected with lipopolysaccharide), and sham injury group (incision to the neck skin and injected with 10 μL of saline solution for normal rats). These five groups were further assigned into three subgroups: day 3 gro up, day 7 group, and day 21 group. To determine how the TLR4/NF-қB signal pathway affects cerebral ischemia–reperfusion injuries, various methods including the Morris water maze, triphenyl tetrazolium chloride (TTC) staining, hematoxylin–eosin (HE) staining, and western blotting were employed i n this study. No neurological deficit was observed in rats from the model control and sham injury groups. A slight neurological deficit was found in the 0.5 h reperfusion gro...
Source: Molecular Neurobiology - Category: Neurology Source Type: research