GSE104599 A regulon enrichment test reveals the activation of major regulators of Mycobacterium tuberculosis gene expression in two models of antibiotic tolerance.

Contributors : Petros C Karakousis ; Dalin Rifat ; William MaternSeries Type : Expression profiling by high throughput sequencingOrganism : Mycobacterium tuberculosisThe acquisition of microbial antibiotic tolerance is suspected to be a major factor in the lengthy duration of current Tuberculosis therapies. We model antibiotic tolerance in vitro by exposing Mycobacterium tuberculosis to two distinct stress conditions: hypoxia and nutrient starvation (PBS). We then measured the bacterial transcriptional response with RNA-seq. We employed a bioinformatics approach to identify important transcriptional regulators of the response to these two stress conditions, which is facilitated by a novel Regulon Enrichment Test (RET) similar to Fisher ’s Exact Test. We also used this approach to analyze previously published gene expression datasets and found that the transcription factors identified from the literature were a subset of those derived from our data. We hypothesize that some of these regulators may play a role in the acquisition a nd maintenance of antibiotic tolerance by Mycobacterium tuberculosis during exposure to stress and provide suggestions for follow-up studies.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mycobacterium tuberculosis Source Type: research