How to transfer a quantitative molecular diagnostic test to multiple qPCR platforms
Quantitative gene expression assays are increasingly used for diagnosis and research, but are often restricted to specific instrumentation. We propose a robust technical and statistical framework that enables transferring of established RT-qPCR assays across qPCR platforms without compromising analytical and clinical validity. The feasibility of our approach on MammaTyper ®, an in vitro diagnostic assay which quantifies breast cancer biomarkers and dichotomizes results according to cut-off points, was tested.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Claudia G ürtler, Mark Laible, Wolfgang Schwabe, Heike Steinhäuser, Xingmin Li, Shujin Liu, Kornelia Schlombs, Ugur Sahin Tags: Technical Advance Source Type: research