A novel compound-primed multiplex ARMS-PCR (CPMAP) for simultaneous detection of common PAH gene mutations

In this study, we introduce a novel compound-primed multiplex ARMS PCR (CPMAP) for simultaneous detection of commonPAH gene mutations. This approach was used successfully for simultaneous identification of six most commonPAH gene mutations in 137 phenylketonuria patients in the Iranian population. A total of six normal and six mutant allele-specific primers and 4 common primers containing a tag sequence of 12 base pair at the 5 ˊ-end were designed and used in two separate optimized multiplex ARMS reactions followed by hot-start PCR. The products were separated and visualized on 3% agarose gel. The CPMAP genotyping data were completely in accordance with the direct sequencing results. The CPMAP suggests a reliable, economi cal and rapid method for simultaneous detection ofPAH point mutations using conventional PCR, which could be applied for diagnosis of other gene mutations.
Source: Metabolic Brain Disease - Category: Neurology Source Type: research