Mp60-15 transcriptome analysis to identify human spermatogonial cells from sertoli cell-only testes

Non-obstructive azoospermia (NOA), particularly Sertoli cell-only (SCO), is the most severe form of male factor infertility and is classically thought to lack germ cells and spermatogenic stem cells (SSCs). However, evidence from microsurgical testicular sperm extraction (micro-TESE) on men with SCO has revealed the presence of focal areas of full spermatogenesis, suggesting the possible presence of SSCs in some patients with SCO lacking mature sperm. To determine whether gene products from SSCs could be detected in SCO, transcriptome analysis using next-generation sequencing (NGS) was performed using human biopsied testicular samples.
Source: The Journal of Urology - Category: Urology & Nephrology Authors: Tags: Infertility: Basic Research & Pathophysiology Source Type: research