A novel method for strict intranasal delivery of non-replicating RSV vaccines in cotton rats and non-human primates.

A novel method for strict intranasal delivery of non-replicating RSV vaccines in cotton rats and non-human primates. Vaccine. 2018 Mar 26;: Authors: Citron MP, Patel M, Purcell M, Lin SA, Rubins DJ, McQuade P, Callahan C, Gleason A, Petrescu I, Knapp W, Orekie C, Chamarthy S, Wen Z, Touch S, Pine M, Fontenot J, Douglas C, Liang X, Espeseth AS Abstract Respiratory syncytial virus (RSV) is the most common viral cause of bronchiolitis and pneumonia in children twelve months of age or younger and a significant cause of lower respiratory disease in older adults. As various clinical and preclinical candidates advance, cotton rats (Sigmodon hispidus) and non-human primates (NHP) continue to play a valuable role in RSV vaccine development, since both animals are semi-permissive to human RSV (HRSV). However, appropriate utilization of the models is critical to avoid mis-interpretation of the preclinical findings. Using a multimodality imaging approach; a fluorescence based optical imaging technique for the cotton rat and a nuclear medicine based positron emission tomography (PET) imaging technique for monkeys, we demonstrate that many common practices for intranasal immunization in both species result in inoculum delivery to the lower respiratory tract, which can result in poor translation of outcomes from the preclinical to the clinical setting. Using these technologies we define a method to limit the distribution of intranasally administere...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research