Detection of renin lineage cell transdifferentiation to podocytes in the kidney glomerulus with dual lineage tracing

Understanding of cellular transdifferentiation is limited by the technical inability to track multiple lineages in  vivo. To overcome this we developed a new tool to simultaneously fate map two distinct cell types in the kidney, and genetically test whether cells of renin lineage (CoRL) can transdifferentiate to a podocyte fate. Ren1cCreER/tdTomato/Nphs1-FLPo/FRT-EGFP mice (CoRL-PODO mice) were generated by cro ssing Ren1c-CreER/tdTomato CoRL reporter mice with Nphs1-FLPo/FRT-EGFP podocyte reporter mice.

Source: Kidney International - March 24, 2018 Category: Urology & Nephrology Authors: Diana G. Eng, Natalya V. Kaverina, Remington R.S. Schneider, Benjamin S. Freedman, Kenneth W. Gross, Jeffrey H. Miner, Jeffrey W. Pippin, Stuart J. Shankland Tags: Technical Notes Source Type: research