Simpson –Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation

This study analyzes SGBS transient phenotypic transformation by quantifying the heterogeneity of LDs, mitochondrial dy namics, and a panel of genes involved in adipocyte differentiation and browning. LDs at 21 days of differentiation were larger than in the previous stages, without any change in the number per cell. The expression of genes such as peroxisome peroxisome proliferator-activated receptor γ, leptin, an d lipase E significantly raised from 0 to 21 days. Adiponectin was significantly upregulated at 14 days of differentiation. Brown-specific marker PR domain containing 16 was highly expressed at D0. The variability of mitochondrial shape and interconnectivity reflects differences in the relative ra tes of fusion and fission, resulting in a significant shift from a networked shape at D7 to a fragmented and swollen one at D14 and D21. The transient phenotype experienced by this cellular model should be considered whether used in studies involving the stimulation of adipocyte browning and could b e an interesting human model to further elucidate the browning process in the absence of any stimulation.
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research