Generation of Mouse for Conditional Expression of Forkhead Box A2 (FOXA2).

Generation of Mouse for Conditional Expression of Forkhead Box A2 (FOXA2). Endocrinology. 2018 Mar 13;: Authors: Wang P, Wu SP, Brooks KE, Kelleher AM, Milano-Foster J, DeMayo FJ, Spencer TE Abstract Forkhead box A2 (FOXA2) is a pioneer transcription factor involved in organ development, function and cancer. In the uterus, FOXA2 is essential for pregnancy and expressed specifically in the glands of the endometrium loss of FOXA2 function occurs during development of endometrial cancer in humans. The present study describes the development of a mouse model for conditional expression of mouse FOXA2. Using a system consisting of a minigene located at the Rosa26 locus, a CAG-S-mFOXA2 allele was generated in embryonic stem cells and subsequently in mice; before activation, the minigene is silent due to a floxed stop cassette inserted between the promoter and the transgene. To validate functionality, mice with the CAG-S-mFOXA2 allele were crossed with progesterone receptor (Pgr)-Cre mice and lactotransferrin (Ltf)-iCre mice that express Cre in the immature and adult uterus, respectively. In immature Pgr-Cre-CAG-S-mFoxa2 mice, FOXA2 protein was expressed in the luminal epithelium (LE), glandular epithelium (GE), stroma and inner layer of the myometrium. Interestingly, FOXA2 protein was not observed in most of the luminal epithelium of uteri from adult Pgr-Cre-CAG-S-mFoxa2 mice, although FOXA2 was maintained in the stroma, glandular epitheliu...
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research